Fig 3. Quantitative expression analysis of Avr3a and virulence towards Rps3a, among the progeny from test cross of P. sojae strains P6497 X [F1-81(ACR10 X P7076)].
Expression of Avr3a was determined by quantitative real-time PCR analysis. Virulence towards Rps3a was determined by a hypocotyl inoculation bioassay. Expression values are a mean, and virulence numbers are a sum of plants killed, derived from two independent biological replicates. Expression of Avr3a was normalized with P. sojae Actin. Parental P. sojae strains P6497 and ACR10 are included for comparison. The correlation (R2) and probability (p) from a regression analysis is also shown.