Table 1. Virulence outcomes and Avr3a transcript detection in F3 and F4 progeny from P. sojae cross ACR10 X P7076.
| F3 progeny1 | F4 progeny1 | Virulence on Rps3a (L83-570)2 | Virulence on rps3a (Williams)2 | Assigned phenotype3 | Avr3a mRNA transcript (RT-PCR)4 |
|---|---|---|---|---|---|
| F3-40 | 60 | 60 | V | - | |
| F4-40(3) | 9 | 15 | NP | - | |
| F4-40(4) | 43 | 45 | V | - | |
| F4-40(5) | 60 | 60 | V | - | |
| F4-40(7) | 60 | 60 | V | - | |
| F3-60 | 60 | 60 | V | - | |
| F4-60(1) | 60 | 60 | V | - | |
| F4-60(2) | 55 | 60 | V | - | |
| F4-60(4) | 53 | 56 | V | - | |
| F4-60(5) | 60 | 60 | V | - | |
| F3-87 | 58 | 60 | V | - | |
| F4-87(1) | 60 | 60 | V | - | |
| F4-87(2) | 55 | 53 | V | - | |
| F4-87(3) | 60 | 60 | V | - | |
| F4-87(4) | 60 | 60 | V | - | |
| F3-83 | 60 | 60 | V | - | |
| F4-83(1) | 60 | 60 | V | - | |
| F4-83(2) | 60 | 60 | V | - | |
| F4-83(3) | 60 | 60 | V | - | |
| F4-83(4) | 59 | 60 | V | - |
1All progeny originally derived from F2 individuals homozygous for the Avr3aP7076 allele
2 Number of dead plants from a total of 60 challenged, representing the sum from two independent virulence assays of 30 plants each.
3 Phenotypes: A, avirulent; V, virulent; NP, non-pathogenic.
4 Symbol (+) positive for Avr3a mRNA, (-) negative for Avr3a mRNA. All samples were positive for control Actin mRNA. Summary of results from two independent biological replicates.