Table 2. Virulence outcomes and Avr3a transcript detection in F4 and F5 progeny from P. sojae cross ACR10 X P7076.
| F4 progeny1 | F5 progeny1 | Virulence on Rps3a (L83-570)2 | Virulence on rps3a (Williams)2 | Assigned phenotype3 | Avr3a mRNA transcript (RT-PCR)4 |
|---|---|---|---|---|---|
| F4-40(7) | 60 | 60 | V | - | |
| F5-40(1) | 20 | 12 | NP | - | |
| F5-40(2) | 59 | 57 | V | - | |
| F5-40(3) | 60 | 59 | V | - | |
| F5-40(4) | 60 | 60 | V | - | |
| F4-60(5) | 60 | 60 | V | - | |
| F5-60(1) | 60 | 60 | V | - | |
| F5-60(2) | 53 | 52 | V | - | |
| F5-60(3) | 55 | 56 | V | - | |
| F5-60(4) | 54 | 47 | V | - | |
| F4-87(1) | 60 | 60 | V | - | |
| F5-87(1) | 60 | 60 | V | - | |
| F5-87(2) | 56 | 58 | V | - | |
| F5-87(3) | 60 | 60 | V | - | |
| F5-87(4) | 45 | 46 | V | - | |
| F4-83(3) | 60 | 60 | V | - | |
| F5-83(1) | 60 | 60 | V | - | |
| F5-83(2) | 60 | 60 | V | - | |
| F5-83(3) | 60 | 60 | V | - | |
| F5-83(4) | 60 | 60 | V | - |
1All progeny originally derived from F2 individuals homozygous for the Avr3aP7076 allele
2 Number of dead plants from a total of 60 challenged, representing the sum from two independent virulence assays of 30 plants each.
3 Phenotypes: A, avirulent; V, virulent; NP, non-pathogenic.
4 Symbol (+) positive for Avr3a mRNA, (-) negative for Avr3a mRNA. All samples were positive for control Actin mRNA. Summary of results from two independent biological replicates.