Table 5. Parentage, virulence outcomes, and Avr3a transcript detection in F₁ hybrids of P. sojae P6497 X ACR10.

F1 progeny	Maternal parent	Virulence on Rps3a (L83-570)1	Virulence on rps3a (Williams)1	Fisher’s test p-value2	Assigned phenotype3	Avr3a mRNA transcript (RT-PCR)4
39	P6497	59	60	0.5	V	+
40	ACR10	60	60	1	V	-
42	ACR10	60	60	1	V	-
45	P6497	56	60	0.05936	V	-
46	P6497	4	60	6.6E-30	A	+
49	P6497	18	53	2.8E-11	A’	+
51	ACR10	59	60	0.5	V	-
52	ACR10	54	56	0.21033	V	-
54	ACR10	45	60	1.1E-05	V’	-
65	P6497	60	60	1	V	-
83	ACR10	17	60	5.1E-19	A’	+
84	P6497	0	60	1E-35	A	+
89	ACR10	12	60	1.6E-22	A’	+
92	P6497	60	60	1	V	-
100	P6497	39	60	5.7E-08	A’	+
106	P6497	0	60	1E-35	A	+
107	P6497	2	60	2E-32	A	+
111	ACR10	7	60	9E-27	A	+
119	P6497	0	60	1E-35	A	+
132	ACR10	5	60	8.5E-29	A	+

¹ Number of dead plants from a total of 60 challenged, representing the sum from two independent virulence assays of 30 plants each.

² Probability value from Fisher’s exact test, on whether the kill rate on test plants (Rps3a) differs from the kill rate on control plants (rps3a).

³ Phenotypes: A, avirulent; V, virulent; A’, incomplete avirulent; V’, incomplete virulent.

⁴ Symbol (+) positive for Avr3a mRNA, (-) negative for Avr3a mRNA. All samples were positive for control Actin mRNA. Summary of results from two independent biological replicates.