Table 6. Segregation analysis for F2 populations developed from virulent and avirulent F1 hybrids of P6497 X ACR10.
| Phenotype1 | F1 | F2 | F2 Phenotype2 | p-value3 | |
|---|---|---|---|---|---|
| Population n | Avirulent mRNA(+) n | Virulent mRNA(-) n | |||
| F1 hybrids silenced for Avr3a and virulent towards Rps3a | |||||
| F1-51 | 25 | 0 | 25 | - | |
| F1-52 | 25 | 0 | 25 | - | |
| F1-92 | 25 | 0 | 25 | - | |
| Total | 75 | 0 | 75 | - | |
| F1 hybrids expressing Avr3a and avirulent towards Rps3a | |||||
| F1-46 | 25 | 19 | 6 | 0.91 | |
| F1-111 | 25 | 21 | 4 | 0.30 | |
| F1-119 | 25 | 16 | 9 | 0.20 | |
| Total | 75 | 56 | 19 | 0.95 | |
1 Selected F1 hybrids from P6497 X ACR10
2 Virulence towards Rps3a and presence of Avr3a mRNA. F2 progeny with avirulent phenotype were positive for Avr3a mRNA transcript (+) and progeny with virulent phenotype were negative for Avr3a mRNA transcript (-), without exception, as determined by RT-PCR.
3 p-value from χ2 analysis for 3:1 segregation