Table 3. Comparison of CT values between two real-time PCR platforms using PowerChek kit.
| EQA phase | Materials | Target | CT (Mean±SD) | P value* | |||
|---|---|---|---|---|---|---|---|
| ABI | N | BioRad | N | ||||
| 1st | 1,000 | upE | 28.2 ± 1.3 | 10 | 29.0±0.8 | 13 | NS |
| ORF1a | 28.3 ± 1.1 | 8 | 28.8±1.1 | 11 | NS | ||
| 100 | upE | 31.5 ± 1.2 | 10 | 32.0±1.0 | 13 | NS | |
| ORF1a | 31.7 ± 0.7 | 10 | 32.4 ±1.4 | 12 | NS | ||
| 10 | upE | 34.5 ± 1.2 | 5 | 36.1±1.2 | 7 | NS | |
| ORF1a | 35.4 ± 1.2 | 5 | 36.9±2.2 | 6 | NS | ||
| 2nd | A | upE | 25.5 ± 0.7 | 14 | 26.3±0.6 | 15 | 0.0011 |
| ORF1a | 26.4 ± 0.7 | 14 | 26.8±0.7 | 15 | 0.0447 | ||
| B | upE | 29.2 ± 0.7 | 14 | 30.0±0.7 | 15 | 0.0035 | |
| ORF1a | 30.0 ± 0.9 | 14 | 30.5±0.8 | 15 | NS | ||
*The comparison of CT values between the two groups was performed by using the Mann-Whitney test because sample sizes of some groups were small.
Abbrviations: EQA, external quality assessment; CT, threshold cycle; NS, not significant.