Table 2. Sequencing statistics of the study.
Patient | Total reads (%) | Human reads (%) | Contigs ≥ 500 bp | H1N1 reads (%)* | H1N1 coverage | HA reads (%)† | NA reads (%)† |
---|---|---|---|---|---|---|---|
P1 | 8,539,246 (100) | 8,344,459 (97.7) | 1,059 | 382 (0.0045) | 6.2 × | 64 (16.8) | 60 (15.7) |
P2 | 7,717,044 (100) | 7,534,324 (97.6) | 1,294 | 216 (0.0028) | 4.2 × | 27 (12.5) | 31 (14.4) |
P3 | 8,406,782 (100) | 8,228,729 (97.9) | 1,473 | 25 (0.0003) | 1.2 × | 7 (28.0) | 6 (24.0) |
P4 | 12,038,410 (100) | 11,649,779 (96.8) | 1,859 | 1,187 (0.0099) | 14.8 × | 109 (9.2) | 168 (14.2) |
P5‡ | - | - | - | - | - | - | - |
P6 | 1,526,958 (100) | 1,474,422 (96.6) | 271 | 1,207 (0.079) | 14.7 × | 93 (7.7) | 123 (11.0) |
*The pandemic H1N1 reference genome used here is the A/Korea/01/2009(H1N1) strain. GenBank accessions are GQ160811-3, GQ131023-6, and GQ132185; †These percentages are for reads only mapped to the pandemic H1N1 reference genome; ‡P5 was excluded from whole-genome sequencing because the quality of extracted RNA was poor.