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. 2015 Jul 9;4:500–509. doi: 10.1016/j.dib.2015.06.022

Table 1.

Analysis of the reproducibility between the three iTRAQ exprements of replicate samples.

IS_113-VS-IS_114
Stage 1: WT_115-VS-MT_118
Stage 2: WT_116-VS-MT_119
Stage 3: WT_117-VS-MT_121
Cut-off at Number Total Coverage (%) Cut-off at Number Total Coverage (%) Cut-off at Number Total Coverage (%) Cut-off at Number Total Coverage (%)
0.10 1126 2906 38.75 0.10 839 3109 26.99 0.10 982 2975 33.01 0.10 810 2702 29.98
0.20 2038 2906 70.13 0.20 1754 3109 56.42 0.20 1938 2975 65.14 0.20 1728 2702 63.95
0.30 2540 2906 87.41 0.30 2407 3109 77.42 0.30 2471 2975 83.06 0.30 2298 2702 85.05
0.40 2752 2906 94.70 0.40 2749 3109 88.42 0.40 2668 2975 89.68 0.40 2514 2702 93.04
0.50 2851 2906 98.11 0.50 2852 3109 91.73 0.50 2760 2975 92.77 0.50 2595 2702 96.04
0.60 2890 2906 99.45 0.60 2883 3109 92.73 0.60 2811 2975 94.49 0.60 2620 2702 96.97
0.70 2903 2906 99.90 0.70 2914 3109 93.73 0.70 2834 2975 95.26 0.70 2633 2702 97.45
0.80 2905 2906 99.97 0.80 2974 3109 95.66 0.80 2864 2975 96.27 0.80 2639 2702 97.67
0.90 2906 2906 100.00 0.90 2995 3109 96.33 0.90 2870 2975 96.47 0.90 2656 2702 98.30
>1.0 2906 2906 100.00 >1.0 3109 3109 100.00 >1.0 2975 2975 100.00 >1.0 2702 2702 100.00
CV (average)=0.17 CV (average)=0.24 CV (average)=0.21 CV (average)=0.19

The table lists the cut-off points (variation), and the corresponding coverage (%) of quantified proteins.

a. “Cut off at” means the variation between the fold change and 1, and the fold change is calculated between two samples in the three experements.

b. “Number” means the number of proteins meet the cut off value.

c. “Total” means the total number of proteins quantified in at least two exprements.

d. “Coverage (%)” is calculated as the “Number” divided by the “Total”,and the higer coverage at a smaller cut off value means the better repeatability.