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Glycosaminoglycan (GAG) content analysis. Immature nucleus pulposus (NP) samples are from discs cultured in different compressive magnitude groups (A), compressive frequency groups (B) and compressive duration groups (C). Data are expressed as the means ± SD, n=3. #: Indicates a significant difference (p<0.05) compared with the control group (p<0.05) between two groups. *: Indicates a significant difference (p<0.05) compared with the compression groups (1.3 MPa, 5 Hz or 8 hour).
