Table 1.

a. Immunization and infection regimens for Indian rhesus macaques that were initially tested with all pools of peptides from antigens.^*

Animal#	Vaccination	Booster	Infection	2nd Infection	3rd Infection
IB78	3D–BCG	MVA-Ag85B	Mtb CDC1551	–	–
GP21, II69	3D–BCG	MVA control	Mtb CDC1551	–	–
HN69, IJ42, HE06, II21	BCG-Aero	–	Mtb	–	–
IJ23, IN85, IM73, CK53, II07	SigH-Aero	–	Mtb	–	–
GI89, II43	–	–	Mtb-Mutant Pools	Mtb-Mutant Pools	SIV-Mac239
HB95	–	–	Mtb-Pool 1,2,3	–	–

b. Immunization and infection regimens for newly tested Indian rhesus macaques.
Animal#	Vaccination	Booster	Infection	Infection	Infection
GK87, CL10	–	–	Mtb CDC1551	–	–
CR57, EA97	BCG-Aero	–	Mtb CDC1551	–	–
FM29, DF44	SigH-Aero	–	Mtb CDC1551	–	–
HK78	3D–BCG	MVA-AG858	Mtb CDC1551	–	–
IL33	BCG-Aero	–	Mtb	–	–

c. Negative control Indian rhesus macaques.
Animal#	Peptide pool tested	Vaccination	Booster	Infection
EA43, FD79, EP16	Pool of 300	None	None	None
IB62, DR27, HE68, FT88, IM14, HT41, CT52, BK01	Pool of 54 and 300	None	None	None

^*Each of the animals was infected with Mtb CDC1551. The animals were either vaccinated and/or immunized or considered a negative control. Immunizations consisted of the recombinant 3D–BCG strain provided by Dr. Doug Kernodle. The animals vaccinated with the 3D–BCG strain were boosted six weeks later with either a control MVA or MVA:Ag85B and then challenged six weeks after the boost with Mtb CDC1551 to deliver 1000 cfu into the lungs of macaques (86). Aerosol vaccination with a single, one time dose of BCG (Danish) (130, 131) as well as Mtb:Δ-sigH (CDC1551 background) [140] were performed in order to deliver ∼5000 cfu of bacilli into the lungs of macaques. The animals were challenged 8 weeks after the single pulmonary vaccination with the identical dose of Mtb CDC1551 (1000 cfu delivered into the lungs of macaques). SIV co-infection was performed 9 weeks after initial low-dose (25–50 cfu delivered to the lungs of macaques) Mtb CDC1551 infection.