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. 2015 Oct 20;7(2):190–198. doi: 10.1111/1759-7714.12313

Figure 2.

figure

Effect of brain and reproductive organ‐expressed (BRE) protein on cisplatin (DDP) resistance in lung cancer cells. (a,b) BRE expression was measured by real‐time polymerase chain reaction (PCR) and western blot in A549 and A549/DDP cells. (c‐e) Ectopic expression of BRE in A549 cells. The messenger ribonucleic acid (mRNA) and protein levels of BRE were examined by real‐time PCR and western blot. Additionally, inhibitory concentration (IC)50 for DDP was measured using cell counting kit‐8 (CCK‐8) assay. (f‐h) Silencing of BRE by small interfering (RNA) in A549/DDP cells. The mRNA and protein levels of BRE were examined by real‐time PCR and western blot. Additionally, IC50 for cisplatin was measured using CCK‐8 assay. Data are reported as mean ± standard deviation for three independent experiments (*P < 0.05, t‐test).