Fig. 3.

Exposure to metoprolol (MTP) did not affect IL-1β-stimulated CGRP release in trigeminal ganglia cell culture. CGRP secretion was determined 45 min after pretreatment with MTP (100 nM and 10 μM) respectively PBS followed by a 24 h exposure to PBS or IL-1β (10 ng/ml). IL-1β stimulation for 24 h led to a significant CGRP release compared to vehicle stimulation (* p = 0.042) which was not altered by pre-treatment with MTP (100 nM or 10 μM) (p > 0.05, compared to pre-treatment with PBS). CGRP levels are shown in mean pg/ml ± SEM, n = 9