Fig. 4.

Pretreatment with metoprolol (MTP) did not affect PGE₂-stimulated CGRP release in trigeminal ganglia cell culture. CGRP secretion was determined 45 min or 24 h after pretreatment with PBS, MTP 100 nM or MTP 10 μM followed by a 4 h exposure to PBS or PGE (10 μM). Stimulation with PGE₂ resulted in an induction of CGRP release (* p < 0.0001 resp. 0.015, compared to treatment with PBS), which was not altered by previous exposure to MTP (100 nM or 10 μM) (p > 0.05, compared to pre-treatment with PBS). CGRP levels are shown in mean pg/ml ± SEM, n = 9