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BHA increases the expression of Gstα1/2 in the liver and small intestine from WT mice. WT and Nrf2-/- mice were given BHA (200 mg/kg) or oil (vehicle) by oral gavage for 3 days. Soluble extracts from the liver (A) and small intestine (SI) (B) were analyzed by Western immunoblotting with antibodies against Gstα1/2. Each lane shows the results for a sample from a single mouse. Actin was used as a loading control.
