Table 1.

Published HCS datasets. A list of HCS studies that have published full datasets—metadata and binary image data—for browsing, query and potential download.

Study	Cell line/organism	Phenotypes measured	Perturbations	Dataset resource(s)	References
Mitocheck	Human HeLa	Cell division defects	Genome-wide siRNA	http://mitocheck.org	[22]
Yeast proteome plasticity	S. cerevisiae	Stress-based protein localization changes	Oxidative stress; starvation	http://jcb-dataviewer.rupress.org/jcb/browse/6203/	[23]
Nuclear body components	Human HeLa	Nuclear body localization	Genome-wide siRNA; ORFeome 5.1	http://jcb-dataviewer.rupress.org/jcb/browse/6852/S152/	[24]
Cell–cell adhesion	Drosophila S2	Adherent cells	Primary genome-wide siRNA	http://jcb-dataviewer.rupress.org/jcb/browse/7555/S202/	[25]
Cell–cell adhesion	Canine MDCK	Adherent cells	Secondary siRNA	http://jcb-dataviewer.rupress.org/jcb/browse/7555/S252/	[25]
SUMO function	S. cerevisiae	Nuclear & cytoplasmic phenotypes	Non-essential mutant library	http://jcb-dataviewer.rupress.org/jcb/browse/6156/S52/	[26]
DNA damage response	S. cerevisiae	Rad52 localisation	Non-essential mutant library	http://jcb-dataviewer.rupress.org/jcb/browse/4608/S1/	[27]
Cytoskeletal structure	Drosophila S2	Actin, microtubule localization	Primary genome-wide siRNA	http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2/	[28]
Cytoskeletal structure	Human Hela	Actin, microtubule localization	Secondary siRNA	http://jcb-dataviewer.rupress.org/jcb/browse/4609/S3/; http://jcb-dataviewer.rupress.org/jcb/browse/4609/S4/	[28]
Sysgro	S. pombe	Cell shape, microtubule defects	Non-essential mutant library	http://sysgro.org	[29]
SH4 Protein targeting	Human HeLa	SH4 domain membrane targeting	Genome-wide siRNA	http://www.ebi.ac.uk/fg/sym/study/B1_SyM	[30]
DNA damage response	Human HeLa; U2OS	53BP1 foci formation	Genome-wide siRNA	http://mitocheck.org/cgi-bin/mtc; http://www.ebi.ac.uk/fg/sym/study/C2_SyM	[31]
ER->Plasma membrane secretion	Human HeLa	tsO45G localization	Genome-wide siRNA	http://mitocheck.org/cgi-bin/mtc; http://www.ebi.ac.uk/fg/sym/study/E1_SyM	[32]
Systems survey of endocytosis	Human HeLa	Transferrin & EGF endocytosis	Genome-wide siRNA/esiRNA	http://endosomics.mpi-cbg.de/	[33]
DNA damage-induced histone ubquintinylation	Human U2OS	GFP-RNF168 localisation to damage loci	Genome-wide siRNA	http://mitocheck.org/cgi-bin/mtc; http://www.ebi.ac.uk/fg/sym/study/G1_SyM	[34]
LINCS	Human various	Apoptosis, proliferation	Mitotic & mTOR inhibitors	http://lincs.hms.harvard.edu/db/	[18]
Broad Bioimage Benchmark	Various	Various	Mutant and siRNA screens	https://www.broadinstitute.org/bbbc/	[17]
DNA damage response	Human Mac2a, K299	Chromosome breaks, translocations	hiBA-FISH; probes that reveal chromosome breaks	http://dx.doi.org/10.5061/dryad.6h7nt	[14]
Cell painting	Human U2OS	Cell phenotype marker localisation	30,000 compounds; various sources	http://www.cellimagelibrary.org/pages/project_20269	[16]