Fig. 1.

A screen of 74 conditions to visualise single iPS cells. List of tested substrate conditions from two combined arrays (Orla and custom made). Columns indicate an arbitrary reference number, name concentration, motifs or residues and source are detailed. Yield refers to number of single cells observed by visual inspection indicative of assay quality. The insets show examples of suboptimal substrates for iPSCs. Very few cells attach when plated on laminin α1 IKVAV (Table 1, n. 11, representative of conditions 1–48) and many clumps and very few single cells are observed when cells are plated on vitronectin PQVTRGDVFTM (Table 1, n. 49, representative of conditions 49–64).