Fig. 6.

Fibronectin conditions affect several phenotypic features. Phenotypic features were interrogated in a series of replicate experiments for each of three fibronectin concentrations in all cells (Fn1; green, Fn5; grey, Fn25, red). (A) Number of cells per well. Min–Max range is shown. (B) Fraction of EdU positive cells over the three fibronectin concentrations. Min–Max range is shown. (C) Inverse mean clump size, an indicator of the propensity of cells to clump. (D) Cell roundness plotted over cell area. Note that cells in higher concentration of fibronectin present a wider range of values. (E–G) Density plots for nucleus area, nucleus roundness and nucleus width to length. The y axe shows the relative fraction of cells. Min–Max range is shown. In all graphs: green, Fn1; grey, Fn5; Orange, Fn25.