Fig. 1.

Binding and spreading of HT1080 (A, B) and Rugli cells (C, D) on collagen films. Cells were added to wells coated with collagen films, cross-linked (see Materials and methods) as indicated. For cell binding assays, 5 × 10⁵ cells/ml were added in the presence of 5 mM of Mg²⁺ or EDTA, during 20 min for HT1080 (A) or 30 min for Rugli (C) before lysis and quantification. For cell spreading assays, 2 × 10⁵ cells/ml were added in the presence of 7.5 mM Mg²⁺ or EDTA during 45 min for HT1080 (B) or 90 min for Rugli (D). Representative images of HT1080 and Rugli cells spread on cross-linked collagen films are shown in (E).