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. 2016 Apr;85:65–77. doi: 10.1016/j.biomaterials.2016.01.044

Fig. 3.

Fig. 3

THP affinity for the α2 I-domain. The recombinant α2 I-domain-GST-fusion protein was incubated at 0.5 μg/ml with THP at 100 μg/ml for 30 min in the presence of Mg2+, with: no peptide (A); GPC(GPP)5GFOGER(GPP)5GPC (B); (GPP)5GFOGER(GPP)5 (C); ES-GFOGER (D); Diazirine-ES-GFOGER (E); Diazirine-ES-GFOGER-Biotin (F); or GPP10 (G). The adhesion of the α2 I-domain to immobilized GPC(GPP)5GFOGER(GPP)5GPC after 1 h was assessed using anti-GST antibody. Control wells were coated with BSA incubated with the α2 I-domain (H).