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. 2016 Apr;85:65–77. doi: 10.1016/j.biomaterials.2016.01.044

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© 2016 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 5 — THP coating on collagen films using UV treatment. Biotinylated THPs were added at 5 μg/ml to wells coated with 100% EDC/NHS cross-linked collagen films and exposed to UV light for 5 min. Following PBS and citrate buffer washings, attached peptides were detected through HRP-Streptavidin binding to Biotin. Pooled means ± SD were calculated from three repeat experiments. Wells were coated with collagen films without peptides (A); in the presence of Diazirine-ES-GFOGER-Biotin (B); or in the presence of ES-GFOGER-Biotin (C). Control wells were coated with BSA in the presence Diazirine-ES-GFOGER-Biotin (D). Diazirine-ES-GFOGER-Biotin followed by UV treatment led to a significant increase in peptide retention compared to all other conditions (p < 0.0001, 2-way ANOVA).