Fig. 7.

HT1080 and Rugli cell binding to peptide derivatized cross-linked collagen films. Diazirine-ES-GFOGER or ES-GFOGER were added to EDC/NHS cross-linked collagen films at 5 μg/ml and exposed to UV light for 5 min. Controls include wells coated with GPC(GPP)₅GFOGER(GPP)₅GPC or BSA. 5 × 10⁵ cells/ml were added to wells in the presence of 5 mM Mg²⁺ or EDTA, lysed and quantitated. A) Rugli cells binding to peptide derivatized 100% EDC/NHS cross-linked collagen films after 30 min; a representative dataset from seven independent experiments is shown (each in triplicate). B) HT1080 cells binding to peptide derivatized 500% EDC/NHS cross-linked collagen films after 20 min; a representative figure from nine independent experiments is shown (each in triplicate).