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. 2016 Feb 26;7:10875. doi: 10.1038/ncomms10875

Figure 6. Lung tissue is a major reservoir for antigen-specific memory B cells.

Figure 6

Analysis of lung-resident B cells 28 days after the last antigen challenge (day 41). (a) Histological analysis of lung tissue showing a peribronchial infiltrate. Antigen-specific B cells (CD45.1+, green) were preferentially found in the proximity of vessels. Br, bronchus, V, vessels. Scale bar, 50 μm, original magnification was × 20. (b) Frequencies and absolute numbers of antigen-specific (CD45.1+) B cells isolated from lymph node (Ln), lung, spleen (Spl) and bone marrow (Bm) on day 41. (c) Characterization of lung-resident antigen-specific B cells showing representative staining for CD38, IgM, IgG1, IgA and the memory markers PD-L2, CD80 and CD73. Representative data from two independent experiments with six mice per experiment are shown. (d) Co-staining of antigen-specific B cells (CD45.1) and FDC (CD21/35) in lung tissue on day 42. Scale bars, 500 μm in the overview and 100 μm in the detail enlargement.