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The central-composite design for optimizing MtEst45 activity.
| X1 | X2 | Y | |
|---|---|---|---|
| 1 | 45.00 | 8.00 | 3.467 |
| 2 | 35.00 | 8.50 | 2.399 |
| 3 | 45.00 | 8.00 | 3.549 |
| 4 | 45.00 | 8.00 | 3.449 |
| 5 | 30.86 | 8.00 | 1.780 |
| 6 | 45.00 | 8.00 | 3.482 |
| 7 | 45.00 | 7.29 | 2.544 |
| 8 | 55.00 | 7.50 | 2.713 |
| 9 | 35.00 | 7.50 | 1.482 |
| 10 | 59.00 | 8.00 | 2.254 |
| 11 | 45.00 | 8.00 | 3.497 |
| 12 | 55.00 | 8.50 | 2.692 |
| 13 | 45.00 | 8.71 | 3.289 |
X1, Temperature (°C); X2, pH; Y, p-nitrophenol (μg).
