Figure 7. Zfp423 expression antagonizes gliomagenesis in a SMAD-dependent manner.

(a) Kaplan–Meyer analysis of survival in mice injected with AstroEGFR*2 overexpressing either Zfp423, Sox2 or both. Reconstitution of Sox2 expression in the context of Zfp423 overexpression reverted the Zfp423-dependent antigliomagenic phenotype (orange line), leading to shorter survival compared with Zfp423 overexpression alone (purple line). Overexpression of both Zfp423 and Sox2 also prolonged survival with respect to Sox2 overexpression alone, indicating mutual antagonism between the two factors. Numbers of animals for each category are indicated in brackets in the caption; P-values were computed with log-rank Mantel–Cox test. (b) Overexpression of a mutant form of Zfp423 lacking the SMAD-binding domain (Zfp423ΔSBD, dark grey curve) fails to prolong survival in mice. Numbers of animals for each category are indicated in brackets in the caption; P-values were computed with log-rank Mantel–Cox test.