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. 2016 Mar 1;7:10727. doi: 10.1038/ncomms10727

Figure 3. The TetR-DOZI– aptamer module provides robust control of gene expression.

Figure 3

(a) RNA-Seq data from PlasmoDB showing the stage-dependent transcription profile for two native parasite promoters, PfCRT (PF3D7_0709000) and PfCAM (PF3D7_1434200). (b) Stage-dependent FLuc expression normalized to RLuc expression (non-regulated internal reference) in the absence and presence of aTc for both PfCRT (top) and PfCAM (bottom) promoter contexts. The regulatory dynamic range (ratio of aTc induced to basal expression) is shown in red for ring-, trophozoite- and schizont-stage parasites. (c) Stage-dependent mean YFP expression in the absence and presence of aTc for both PfCRT (top) and PfCAM (bottom) promoter contexts. Both the FLuc and YFP reporters are controlled by a single TetR aptamer in the 5′-UTR and 10 tandem TetR aptamers in the 3′-UTR. Fold-induction values were not calculated from the fluorescence reporter expression data as the signal to noise is relatively low, and in the repressed state the fluorescence signal is effectively at the autofluorescence background level. Data shown are the mean±s.d. from biological triplicates, and are representative of two to four independent experiments. *P<0.001 by t-test. NS=Not significant.