Skip to main content
. 2016 Mar 1;7:10727. doi: 10.1038/ncomms10727

Figure 4. Multi-dimensional synthetic control of translation achieves significant reduction in leaky expression.

Figure 4

(a) FLuc expression levels in the absence and presence of aTc normalized to RLuc expression (non-regulated internal reference) when TetR and TetR-DOZI are used to regulate FLuc expression via aptamers positioned within the 5′-UTR, 3′-UTR or both as shown. (b) Fold change in maximal FLuc expression upon induction (+0.5 μM aTc) and in basal FLuc expression (−aTc) is shown relative to the context in which TetR regulates FLuc expression via a single aptamer in the 5′-UTR. Data shown are the mean±s.d. from biological triplicates, and are representative of two to four independent experiments. *P<0.001 by t-test. NS=Not significant.