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. 2016 Mar 1;7:10851. doi: 10.1038/ncomms10851

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(a) Larval wing (left) and haltere (right) discs showing the expression pattern of the nub-Gal4 with the reporter UAS-His2ADsRed. Images are a full projection of the disc showing all nuclei in green and the nub-Gal4>UAS-His2ADsRed in red. (b) Expression of nub-Gal4 at 28 h APF when the wing has reached the ‘definitive stage' where it resembles the adult wing in every respect but size. nub-Gal4 expression includes all wing blade cells, but stops at the junction with the hinge. (c–g) Adult wings from CONT (c), N800 (d), N712 (e) and N678 (f) genotypes, each image representing the wing closest to the mean PC1 coefficient for the genotype. (g) shows the wing with the most extreme PC1 coefficient from the N678 genotype. (c,g) highlight the fifteen landmarks used in our principal component analysis. With increasing morphometric severity, landmarks at the tip of the wing move distally, while anterior and posterior landmarks move closer to the medial axis resulting in a more tapered wing with higher aspect ratio. Scale bar, 1 mm (c–g).