Table 1. Morphological and performance summary for the control group flies.
| Flight variable | Control group mean±s.d.* | df | F† | P-value† |
|---|---|---|---|---|
| Morphology | ||||
| Mass (mg) | 1.18±0.12 | 3,78 | 1.37 | 0.257 |
| Principal Component 1 | — | 3,74 | 922.56 | <0.001 |
| Aspect ratio (single wing mean) | 2.52±0.02 | 3,74 | 630.08 | <0.001 |
| Relative asymmetry (%) | 0.67±0.55 | 3,71 | 5.93 | 0.001 |
| Flight performance | ||||
| Velocity maximum (m s−1) | 1.60±0.16 | 3,78 | 14.44 | <0.001 |
| Velocity mode (m s−1) | 0.71±0.33 | 3,78 | 0.07 | 0.977 |
| Tangential acceleration maximum (m s−2) | 6.60±1.23 | 3,78 | 17.51 | <0.001 |
| Tangential acceleration minimum (m s−2) | −7.68±2.83 | 3,78 | 12.77 | <0.001 |
| Turn radius minimum (mm) | 13.2±5.9 | 3,78 | 8.22 | <0.001 |
| Turn radius mode (mm) | 85.9±28.5 | 3,78 | 6.31 | <0.001 |
| Turn rate maximum (deg s−1) | 1427±378 | 3,78 | 9.03 | <0.001 |
| Turn rate mode (deg s−1) | 112±21 | 3,78 | 4.59 | 0.005 |
*Flight performance means±s.d. for, CONT flies: n=21 individuals. There were 101 flights in the control group in total.
†Analysis of variance test for modulation of flight performance across strains of flies with increasing phenotypic severity (n=85). Significant results are highlighted in bold and reveal a difference if any one of the experimental groups vary with respect to CONT. For example, when testing for relative asymmetry, only N678 flies differed from CONT. We used the False Discovery Rate method to control the expected proportion of false positives at the 5% level and reduce the likelihood of Type 1 errors42,43.