Figure 3.

Hd-Sb reduce the activation of Akt pathway and the expression of antiapoptotic proteins. 5637 (a) and T24 (b) were treated with different concentration of Hd-Sb for 48 h and the proteins phosphorylated Akt, Akt, Mcl-2, Bcl-2, activated caspase-3, and β-actin were detected by Western blot. ((c) and (d)) The pixel density of these proteins was normalized to β-actin and the relative values from three independent experiments were presented by histogram. (The asterisks represent the value of P, where ^∗ P < 0.05, ^∗∗ P < 0.01, and ^∗∗∗ P < 0.001.)