Figure 4.

Hd-Sb decreases miR-155 expression and anti-miR-155 promotes cell apoptosis through Akt pathway. (a) 5637 and T24 cells were treated with 1 mg/mL Hd-Sb and the expression of miR-155 was determined using RT-PCR for 48 h. The cell viability (b) and cell apoptosis (c) were measured using MTT assay and FACS analysis. (d) 5637 and T24 cells were transfected with miR-155 inhibitor or control siRNA for 48 h and the phosphorylated Akt, Akt, and β-actin were detected using Western blot. (The asterisks represent the value of P, where ^∗ P < 0.05, ^∗∗ P < 0.01, and ^∗∗∗ P < 0.001.)