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. 2016 Feb 17;2016:5317630. doi: 10.1155/2016/5317630

Figure 3.

Figure 3

G-CSF combined with HHES promoted angiogenesis in the wound areas. (a) Typical cytokines relative expression of IL-6, IL-10, TGF-β, TNF-α, and VEGF mRNA during the wound healing. (b) VWF and Ki67 IF staining of the wound areas on day 9. Three-color fluorescent images of the vasculature in the frozen sections of the wound sites that were stained for Ki67 (green), VWF (red), and hoechst33342 (blue). (c) The mean percentages of the proliferative blood vessels coexpressing VWF (red) and Ki67 (green) at the wound sites. The data are shown as the means ± SEM. ∗ P < 0.05 versus the blank in the same group; # P < 0.05 versus normal saline in the same group. § P < 0.05 versus Unres/G-CSF in the same group. Unresuscitated rats with G-CSF (Unres/G-CSF), induction of hemorrhagic shock without resuscitation but with G-CSF injection. The white dashed frame indicated magnification typical VWF/Ki67 double staining areas. Scale bar = 50 μm.