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. 2016 Feb 17;2016:4041827. doi: 10.1155/2016/4041827

Figure 2.

Figure 2

Experimental data of metabolic profiling of adrenal steroid hormones and the mass balance. Concentrations of steroid hormones secreted from NCI-H295R cells in the culture medium at 72 h after stimulation were compared with the untreated condition and the stimulated condition by 50 nM ACTH, 20 μM forskolin, and 100 nM angiotensin II (a). Net molecular amounts including cholesterol and steroid hormones in the culture medium and intracellular space are plotted at five time points (0, 8, 24, 48, and 72 h after stimulation) under the untreated condition (b) and the stimulated condition (c). In the bar graphs, dark and light bars indicate the amount of cholesterol and adrenal steroids, respectively. All data are shown as the mean ± SD (N = 4). ∗ p values corrected by the familywise error rate <0.01. PREG: pregnenolone, HPREG: 17α-hydroxypregnenolone, DHEA: dehydroepiandrosterone, PROG: progesterone, HPROG: 17α-hydroxyprogesterone, DIONE: androstenedione, DCORTICO: 11-deoxycorticosterone, DCORT: 11-deoxycortisol, CORTICO: corticosterone, CORT: cortisol, ALDO: aldosterone, and TESTO: testosterone.