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Overexpression of 5-HT biosynthesis rate-limiting enzyme, tryptophan hydroxylase (Trh), in serotonergic neurons. (a) Trh overexpression reduces body wall contractions. (b) Trh overexpression significantly decreased mouth hook movements as an indicator for feeding behavior. The flies were raised at 23°C as well as at 29°C to manipulate the expression level of transcriptional activator Gal4. Student's t-test was performed to carry out comparison between groups.
