Figure 6.

Manipulation of the 5-HTergic system. (a) Activation of serotonergic neurons, by using thermogenetic approach (TrpA1 channels), during early stages of development modulates locomotion behavior in later stage of development. RNA interference- (RNAi-) directed 5-HT1B receptor knockdown during early stage of development significantly reduced the locomotion behavior in third-instar larvae. (b) Activation of serotonergic neurons increased the feeding behavior. RNAi-directed 5-HT1B and 5-HT2A receptors significantly reduced the feeding behavior in third-instar larvae. Student's t-test was performed to carry out comparison between groups. Data presented as mean ± SEM.