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Q-PCR analysis of proteoglycan biosynthesis enzyme gene expression in hNSC H9 cells and nhNPCs. Relative expression in hNSC H9 cells and nhNPCs of: (A) HS chain synthesising and modifying enzymes; (B) HS chain N-deacelylating/N-sulfating enzymes; (C) HS chain sulfating enzymes; and (D) CS chain sulfating enzymes. Relative expression normalised to 18 S, error bars=SD, statistical significance: ⁎p<0.05, ⁎⁎p<0.01, ⁎⁎⁎p<0.001.
