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. 2016 Feb 19;7:206–215. doi: 10.1016/j.dib.2016.02.030
Subject area Cell biology
More specific subject area Human neural stem cell (hNSC) and human neural progenitor cell (hNPC) marker characterisation
Type of data Text file, graphs and immunofluorescence images
How data was acquired in vitro culture/expansion and phase-contrast fluorescence microscopy data for phenotypic analysis was obtained on an Olympus IX81 inverted fluorescent microscope via Volocity Imaging package; raw Q-PCR data was obtained on Applied Biosystems 7900HT Fast Real-Time PCR system
Data format Analysed
Experimental factors hNSC H9 and nhNP cells were cultured under basal medium conditions
Experimental features hNSC H9 cells (Thermo Fisher) were cultured as a monolayer and nhNP cells (Lonza) were cultured as neurospheres in standard maintenance medium provided by the manufacturer. RNA was harvested and transcribed to cDNA and gene expression of a panel of 49 genes examined by Q-PCR. Specific neural cell lineage markers were further detected through immunofluorescence (IF)
Data source location Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland Australia
Data accessibility Data is provided in this article