Subject area |
Cell biology |
More specific subject area |
Human neural stem cell (hNSC) and human neural progenitor cell (hNPC) marker characterisation |
Type of data |
Text file, graphs and immunofluorescence images |
How data was acquired |
in vitro culture/expansion and phase-contrast fluorescence microscopy data for phenotypic analysis was obtained on an Olympus IX81 inverted fluorescent microscope via Volocity Imaging package; raw Q-PCR data was obtained on Applied Biosystems 7900HT Fast Real-Time PCR system |
Data format |
Analysed |
Experimental factors |
hNSC H9 and nhNP cells were cultured under basal medium conditions |
Experimental features |
hNSC H9 cells (Thermo Fisher) were cultured as a monolayer and nhNP cells (Lonza) were cultured as neurospheres in standard maintenance medium provided by the manufacturer. RNA was harvested and transcribed to cDNA and gene expression of a panel of 49 genes examined by Q-PCR. Specific neural cell lineage markers were further detected through immunofluorescence (IF) |
Data source location |
Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland Australia |
Data accessibility |
Data is provided in this article |