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. 2016 Mar 2;7:67. doi: 10.3389/fimmu.2016.00067

Figure 2.

Figure 2

Characterization of CCGAD65-induced CD4+ T cells. (A) The proliferative response of CCGAD65-induced CD4+ T cells (pooled from three previously CCGAD65-immunized mice) to either CCGAD65 peptide (continuous line) or the natural counterpart (WTGAD65, dashed line) was assayed after 72 h in cocultures with Mitomycine C®-treated T-cell-depleted splenocytes loaded with the indicated peptide concentrations. 3H-thymidine was added for the last 12 h of culture. Error bars represent 1 SD. Data from one experiment representative of three experiments performed in triplicate wells. (B) CD4+ T cells induced either with WTGAD65 peptide (left panels) or CCGAD65 peptide (right panels) were analyzed by FACS at day 10 of stimulation. Results are from one experiment representative of three independent experiments. Additional information can be found as Figures S3 and S4 in Supplementary Material.