Figure 5.

NOD mice transferred with cCD4⁺ T cells induced by CCGAD65. Naive mice were passively transferred at 6 weeks of age with cCD4⁺ T cells (2 × 10⁵) obtained from mice preimmunized with CCGAD65 peptide (from 8 to 12 weeks and sacrificed at 15 weeks) and expanded in vitro with CCGAD65. Mice were followed up with weekly blood glucose measurements until 40 weeks of age. (A) Diabetes-free survival curves are shown for mice transferred with CCGAD65-generated CD4⁺ T cells (n = 11), compared to the group transferred with AAGAD65-generated CD4⁺ T cells (n = 12) as indicated, *P < 0.05 (Mantel–Cox log-rank test). (B) Pancreatic sections of normoglycemic mice at 40 weeks of age transferred either with CCGAD65-induced CD4⁺ T cells or AAGAD65-induced CD4⁺ T cells were stained with H&E and insulitis was scored for at least 40 islets per mouse. Left panel: vertical axis represents insulitis index for each mouse, line indicates mean per group, treated group (n = 8) was compared to control group (n = 4). Statistical significance was calculated using two-tailed Mann–Whitney, **P < 0.01. Right panel: vertical axis represents percentage of insulitis-free islets per mouse, line indicates mean per group. Treated group was compared to control, **P < 0.01 (two-tailed Mann–Whitney test).