Figure 6.

DHS silencing causes hypersensitivity to ABA. (A) 4-day-old wild type and siDHS-K plants grown in vitro under long-day conditions with or without dexamethasone were transferred to medium with 10 μM ABA for 10 additional days when pictures were taken (left) and the growth of the main root of at least 5 individual plants of each transgenic line and treatment was measured (right). (B) Seedling establishment was estimated by counting wild type and siDHS-K plant seedlings with open healthy leaves after sowing in the absence or in the presence of 0.3 μM ABA with or without dexamethasone. Left panel shows a representative picture and the statistical quantification with at least 6 individual plants of each genotype and treatment is shown on the right. (C) Germination percentage was determined by counting the number of seedlings able to culminate radicle protrusion after sowing in the absence or in the presence of 2 μM ABA with or without dexamethasone. The statistical analysis performed in all the cases was one-way anova test. Asterisks indicate significant differences (p < 0.05) with respect to wild type plants (one asterisk) or with respect to wild type plants and mock-treated plants of the same genotype (two asterisks).