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. 2016 Mar 2;3:5. doi: 10.3389/fcvm.2016.00005

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Copyright © 2016 Boulaksil, Bierhuizen, Engelen, Stein, Kok, van Amersfoorth, Vos, van Rijen, de Bakker and van Veen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cx43 dephosphorylation. (A) Typical examples of immunolabeling with anti-Cx43 and anti-non-phosphorylated Cx43 (Cx43-NP) along with merged photographs of both labelings from LV of two different TAC+ rats (upper two rows). Lowest row shows a higher magnification of a border zone between normal Cx43 expression and diminished Cx43 with substitution by Cx43-NP. (B) Western blot of non-phosphorylated Cx43 expression (Cx43-P0) in the same LV myocardial samples as in Figure 4C. (C) Quantifications of Cx43-NP expression, normalized to sham. Scale bar = 100 μm. *P < 0.05 vs. sham.