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. 2016 Mar 2;7:228. doi: 10.3389/fpls.2016.00228

Figure 4.

Figure 4

Overexpression of OsCEP6.1 negatively affected the vegetative growth of rice. (A) Comparison of the root length and shoot height between the control and seedlings with synthetic CEP at the 8-day-old stage. The growth of shoot and root was significantly repressed by synthetic CEP peptide, by 17 and 20%, respectively. Bar = 1 cm. The values are means ± SD (n = 18), **P < 0.01. (B) Quantitative RT-PCR analysis revealed the high expression level of OsCEP6.1 in six overexpression lines at the T0 generation, particularly lines #5 and #6. (C) Comparison of the root length and shoot height of the transgenic lines (#5) and control at the 14-day-old seedlings. The growth of shoot and root was significantly repressed by approximately 25% in the transgenic line compared with the control. Bar = 2 cm. The values are means ± SD (n≥10), **P < 0.01. (D) Overexpression of OsCEP6.1 leads to phenotypic variation at the mature stage including plant stature and panicle architecture. Bar = 20 cm. (E) OsCEP6.1 overexpression lines exhibited significantly fewer tillers and shorter height compared with those of the control. (F) Both the length and width of flag leaves were significantly smaller in the transgenic line (#5 and #6) than those in the control. Bar = 10 cm. The values are means ± SD (n≥12), **P < 0.01.