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. 2016 Mar 2;7:228. doi: 10.3389/fpls.2016.00228

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Copyright © 2016 Sui, Wang, Li, Zhang, Li, Xu, Xing, Ni and Xin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantitative RT-PCR analysis revealed that eight cell-cycle-related genes, including five G1/S-phase genes (CDKA1, CAK1, CAK1A, CYCT1, and CYCD4) and three G2/M-phase genes (CYCIaZm, CDKB, and CYCB2.1), were significantly down-regulated in the transgenic line compared with the control. The expression levels were determined using 6–8 cm young panicles from at least five plants, the values are means ± SD, ^*P < 0.05, ^**P < 0.01.