Fig. 5.

The modulation of key components of Ucp1 regulation in iBAT in response to CA treatment at thermoneutrality. Protein phosphorylation levels of PKA (RIIβ) (A), p38 MAPK (B), cAMP response element-binding protein (CREB; C), and AMP-activated protein kinase (AMPK; D) in iBAT in WT and Ucp1-KO mice fed a HFD or HFD supplemented with CA. Fields with targeted proteins and β-actin in immunoblots were cropped at appropriate band size. All samples were derived at the same time and processed in parallel. Data are presented as means ± SE. Differences between groups were analyzed by Mann-Whitney test. *P < 0.05 and **P < 0.01; n = 5–6.