Fig. 1.

Generation of Clq/TNF-related protein (Ctrp3)-knockout (KO) mice. A: schematic showing the strategy for generating Ctrp3-KO mice by targeted deletion of exon 4/intron 4 of the mouse Ctrp3 gene and replacement with a lacZ reporter and a neomycin resistance cassette. The green circle represents a splice acceptor (SA) site, and the red triangle represents the Flp recombinase target (FRT) site recognized by Flp recombinase. B: genotyping results indicate the successful generation of Ctrp3-wild-type (WT) (+/+), heterozygous (+/−), and homozygous KO (−/−) alleles using the primer set (→ and ←) indicated in A. C: PCR analysis indicates the absence of detectable Ctrp3 mRNA in KO mice. BacVec, bacterial artificial chromosome vector; 5′-UTR, 5′-untranslated region.