Fig. 8.

Analysis of viability of SH-SY5Y cells after treatment with conditioned microglia supernatant. Microglia (BV2 cells) were pretreated with 2-ME for 24 h, washed twice with PBS, and treated in the presence or absence of LPS. After 24 h, the medium was transferred onto SH-SY5Y cells. The viability of SH-SY5Y cells after 24 h of incubation with the conditioned medium was assessed using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Results are presented as means ± SE (n = 3). *Significant reversal of cell toxicity by 2-ME.