Fig. 9.

A: representative Western blot demonstrating the presence of ERβ, but not ERα, in microglia and the modulatory effects of 10, 100, and 1,000 nmol/l of E₂ on the expression of ERβ. B–G: modulatory effects of ER agonists and antagonist on FBS-induced DNA synthesis (B, D, and F) and cell number (C, E, and G). Antiproliferative effects of 100 nmol/l E₂ were attenauted by ICI-182,780 (5 μmol/l, ERα/β antagonist; B and C). Moreover, the antiproliferative effects of E₂ were mimicked by DPN (ERβ agonist; D and E) but not by 4,4′,4′′-(4-propyl-[1H]-pyrazole-1,3,5-triyl)trisphenol (PPT; ERα agonist; D and E). The effects of 100 nmol/l estradiol were not blocked by 1,3-bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy)phenol]-1H-pyrazole (MPP; ERα antagonist, 5 nmol/l; F and G). Results are presented as means ± SE (n = 4). *P < 0.05 vs. C; §P < 0.05 between indicated treatments. NS, not significant.