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. Author manuscript; available in PMC: 2016 Mar 2.
Published in final edited form as: Methods. 2015 Sep 7;96:40–45. doi: 10.1016/j.ymeth.2015.09.006

Fig. 1.

Fig. 1

Fluorogen activating protein (FAP) technology. (A) Cartoon of the FAP-CFTR construct. In order to express the fluorogen activating protein (FAP) extracellularly on CFTR, a FAP/PDGFR-B transmembrane domain was fused to the N-terminus of CFTR. (B) The structure of MG-B-TAU dye. (C) Membrane-impermeant MG-B-TAU (red dots) binds to surface localized FAP and fluoresces when excited with 640 nM laser light. Internalized FAP is not labeled and unbound MG-B-TAU does not fluoresce.