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. Author manuscript; available in PMC: 2016 Mar 2.
Published in final edited form as: Methods. 2015 Sep 7;96:40–45. doi: 10.1016/j.ymeth.2015.09.006

Fig. 3.

Fig. 3

Determination of Z′-factor. The Z0-factor for the assay was determined by treating one half of a 96-well plate containing 293A cells stably transfected with FAP-F508del CFTR with 2 μM VX-809 and the other half with DMSO as a control for 24 h before adding Hoechst 33342 and MG-B-TAU followed by imaging. The resulting values are plotted and the Z′-factor calculated. The experiment was repeated twice with similar results.