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. Author manuscript; available in PMC: 2016 Mar 2.
Published in final edited form as: Methods. 2015 Sep 7;96:40–45. doi: 10.1016/j.ymeth.2015.09.006

Fig. 4.

Fig. 4

DMSO and time dependence of MG-B-TAU signal. (A) The DMSO dependence of the assay was determined by treating 293A cells stably transfected with FAP-F508del CFTR with the indicated concentration of DMSO plus/minus 2 μM VX-809 for 24 h before adding Hoechst 33342 and MG-B-TAU followed by imaging. The resulting values are plotted and the Z′-factors calculated. (B) The time dependence of fluorescence signal intensity after MG-B-TAU addition was determined by treating 293A cells stably transfected with FAP-F508del CFTR with 2 μM VX-809 for 24 h before adding Hoechst 33342 and MG-B-TAU followed by imaging at the indicated time points. The experiments were repeated with similar results.