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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Radiat Res. 2016 Jan 20;185(2):134–150. doi: 10.1667/RR14035.1

FIG. 6.

FIG. 6

Quantitation of increased mitochondria in Fancd2−/− mouse oral cavity tissue and in cell lines. Western blot analysis for mitochondrial proteins Sod2, Tfam and Cox4 was performed with oral cavity cells, TC-1 tumors or bone marrow stromal cells (19). Freshly removed oral cavity cells from Fancd2+/+, Fancd2+/−and Fancd2−/− mice as well as explanted TC-1 tumors from each genotype were lysed and electrophoresed, and proteins were transferred to nitrocellulose membrane and probed for protein expression using antibodies to Sod2, Tfam and Cox4. Densitometry was performed to quantitate protein expression using LabWorks Image Acquisition and Analysis Software. Protein expression was standardized by comparing the densitometry results for Sod2, Tfam and Cox4 to Gapdh. Protein expression is shown as fold increase compared to oral cavity Fancd2+/+ cell expression, which was set as 1.0. There was a significant increase in Fancd2−/− mitochondrial proteins (P < 0.05) in the bone marrow stromal cells and oral cavity cells compared with corresponding Fancd2+/+ cells (*). Protein expression for explanted TC-1 tumor cells was independent of mouse genotype of the oral cavity microenvironment.